| Spartak Police nad Metuji vs Letohrad 04 oct | 3.5 | 1.72 / 1.89 | 4 | over |
| Sokol Hostoun vs Rapid Psary 04 oct | 3.5 | 2.03 / 2.00 | 2 | under |
| Turnov vs Chrudim II 04 oct | 4.5 | 2.06 / 1.86 | 5 | over |
| Jiskra Ústí nad Orlicí vs Kolín 04 oct | 3.5 | 2.24 / 2.21 | 3 | under |
| FC Znojmo vs AFC Humpolec 04 oct | 3.5 | 2.15 / 2.15 | 2 | under |
| Strani vs Šternberk 04 oct | 3.5 | 1.64 / 1.64 | 4 | over |
| Zdas Zdar nad Sazavou vs Sokol Tasovice 04 oct | 2.5 | 1.58 / 1.58 | 1 | under |
| TJ Sokol Lanzhot vs SFK Holešov 04 oct | 3.5 | 2.24 / 2.09 | 2 | under |
| Kravare vs Pusta Polom 04 oct | 3.5 | 1.84 / 1.84 | 3 | under |
| Velka Bites vs Sokol Bedrichov 04 oct | 3.5 | 1.89 / 1.84 | 9 | over |
| Chotebor vs Slovan Havlickuv Brod 04 oct | 2.5 | 1.69 / 1.69 | – | – |
| Frydek-Mistek II vs Zabreh 04 oct | 3.5 | 2.08 / 2.08 | 3 | under |
| Tatran Vsechovice vs Prostejov II 04 oct | 3.5 | 1.84 / 1.68 | 3 | under |
| Kozlovice vs MSK Břeclav 04 oct | 3.5 | 2.13 / 2.19 | 2 | under |
| Chlumec NC vs Hlinsko 04 oct | 3.5 | 1.91 / 1.91 | 2 | under |
| FC Karlovy Vary vs Meteor Prague VIII 03 oct | 3.5 | 2.05 / 2.00 | 5 | over |
| SK Kosmonosy vs Slavia HK 03 oct | 3.5 | 1.88 / 1.66 | 3 | under |
| Dosta Bystrc vs Pelhrimov 03 oct | 3.5 | 2.29 / 2.22 | 3 | under |
| SK Krumvir vs Hluk 03 oct | 3.5 | 2.01 / 2.01 | – | – |
| Nové Sady vs Brumov 03 oct | 3.5 | 2.14 / 2.14 | 5 | over |
| Trebic vs Tatran Bohunice 03 oct | 2.5 | 1.61 / 1.61 | – | – |
| Prepere vs TJ Svitavy 03 oct | 3.5 | 1.77 / 1.77 | 4 | over |
| Sparta Brno vs Zdirec nad Doubravou 03 oct | 3.5 | 2.21 / 2.21 | 4 | over |
| Kurim vs FC Velke Mezirichi 03 oct | 3.5 | 1.94 / 1.94 | 3 | under |
| Slavoj Ceský Krumlov vs Rokycany 03 oct | 3.5 | 2.26 / 2.26 | 4 | over |
| Ujezd Praha 4 vs Slovan Velvary 03 oct | 3.5 | 1.75 / 1.76 | 3 | under |
| Brandýs nad Labem vs Slavoj Vysehrad 03 oct | 3.5 | 1.79 / 1.79 | 7 | over |
| Vratimov vs Bridlicna 03 oct | 3.5 | 1.85 / 1.86 | 3 | under |
| Petrin Plzen vs Spartak Pribram 03 oct | 3.5 | 2.27 / 2.27 | 7 | over |
| FC Hlucin vs Nový Jičín 02 oct | 3.5 | 2.24 / 2.01 | 2 | under |
| Hluboka nad Vltavou vs Krimice 02 oct | 2.5 | 1.64 / 1.64 | 2 | under |
| TJ Dvůr Králove nad Labem vs Prepere 27 sept | 3.5 | 1.89 / 1.89 | 1 | under |
| Kolín vs Turnov 27 sept | 3.5 | 2.06 / 1.97 | 3 | under |
| FK Junior Strakonice vs Slavoj Ceský Krumlov 27 sept | 3.5 | 2.06 / 1.97 | 5 | over |
| Milevsko vs Spartak Sobeslav 27 sept | 3.5 | 1.98 / 2.06 | 3 | under |
| TJ Svitavy vs Trutnov 27 sept | 3.5 | 2.25 / 2.25 | 4 | over |
| Bridlicna vs Šumperk 27 sept | 3.5 | 2.31 / 2.31 | – | – |
| Zdirec nad Doubravou vs Dosta Bystrc 27 sept | 2.5 | 1.58 / 1.58 | – | – |
| Pelhrimov vs Kurim 27 sept | 3.5 | 2.21 / 2.21 | – | – |
| Sokol Tasovice vs Trebic 27 sept | 2.5 | 1.60 / 1.60 | 2 | under |
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