| SK Benatky nad Jizerou vs Chrudim 04 oct | 3 | 1.97 / 1.97 | 6 | over |
| Caslav vs Velke Hamry 04 oct | 3 | 1.78 / 1.81 | 3 | push |
| Varnsdorf vs Slovan Liberec II 04 oct | 3.5 | 1.79 / 1.79 | 2 | under |
| Varnsdorf vs Slovan Liberec II 04 oct | 3.5 | 1.78 / 1.73 | 2 | under |
| Slavia Prague III vs Academia Povltavska 04 oct | 3 | 1.69 / 1.74 | 4 | over |
| Sokol Brozany vs SK Zapy 04 oct | 3 | 1.81 / 1.81 | 0 | under |
| Teplice II vs Neratovice-Byskovice 04 oct | 3 | 1.81 / 1.91 | 2 | under |
| Viktoria Plzen II vs Dukla Prague II 04 oct | 3.5 | 2.06 / 1.66 | 3 | under |
| Bohemians 1905 II vs FC Pisek 04 oct | 3 | 1.86 / 1.81 | 6 | over |
| Hradec Kralove II vs Mlada Boleslav II 04 oct | 3 | 1.93 / 1.93 | 1 | under |
| Zbrojovka II vs Blansko 04 oct | 3.5 | 1.76 / 1.81 | 2 | under |
| Unicov vs Polanka nad Odrou 04 oct | 3 | 1.91 / 1.81 | 4 | over |
| Karvina II vs Zlin II 04 oct | 3 | 1.71 / 1.63 | 1 | under |
| Admira Prague vs Jiskra Domažlice 04 oct | 3.5 | 2.08 / 1.88 | 8 | over |
| Kraluv Dvur vs Sparta 2 Prague 03 oct | 3 | 1.78 / 1.78 | – | – |
| Dynamo Ceske Budejovice vs Benešov 03 oct | 3.5 | 2.01 / 1.95 | 3 | under |
| Banik Sous vs Jablonec II 03 oct | 3 | 1.97 / 1.97 | 4 | over |
| Pribram II vs Aritma Prague 03 oct | 3 | 1.91 / 1.97 | 2 | under |
| FC Frydek Mistek vs Vrchovina 03 oct | 2.5 | 1.66 / 1.68 | 6 | over |
| CSK Uherský Brod vs Havirov 03 oct | 3 | 1.91 / 1.71 | 4 | over |
| Hlubina vs Hodonín 03 oct | 3 | 2.01 / 2.01 | 5 | over |
| Vsetin vs 1. Slovacko II 03 oct | 2.5 | 1.64 / 1.68 | 1 | under |
| Motorlet vs Loko Praha 03 oct | 3 | 2.06 / 2.06 | 3 | push |
| Sigma 2 Olomouc vs MFK Vitkovice 03 oct | 3 | 2.06 / 1.71 | 3 | push |
| Pardubice II vs Horni Redice 03 oct | 3.5 | 2.01 / 2.01 | – | – |
| Hranice vs Artis Brno II 02 oct | 2.5 | 1.61 / 1.61 | 2 | under |
| Hranice vs Artis Brno II 02 oct | 3 | 1.97 / 2.06 | 2 | under |
| SK Zapy vs Banik Sous 27 sept | 2.5 | 1.66 / 1.66 | 2 | under |
| SK Zapy vs Banik Sous 27 sept | 3 | 2.06 / 2.25 | 4 | over |
| Polanka nad Odrou vs FC Frydek Mistek 27 sept | 2.5 | 1.76 / 1.79 | 0 | under |
| Polanka nad Odrou vs FC Frydek Mistek 27 sept | 2.5 | 1.78 / 1.79 | 1 | under |
| Dukla Prague II vs Bohemians 1905 II 27 sept | 3.5 | 2.09 / 2.00 | 4 | over |
| Havirov vs Unicov 27 sept | 2.5 | 1.64 / 1.66 | 1 | under |
| Jablonec II vs Hradec Kralove II 27 sept | 2.5 | 1.58 / 1.58 | 1 | under |
| Dukla Prague II vs Bohemians 1905 II 27 sept | 3.5 | 2.06 / 2.06 | 4 | over |
| Havirov vs Unicov 27 sept | 3 | 2.01 / 1.91 | 2 | under |
| Sparta 2 Prague vs Viktoria Plzen II 27 sept | 3.5 | 2.06 / 2.06 | 0 | under |
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