| Tonbridge Angels vs Torquay United 03 oct | 3 | 1.97 / 1.86 | 1 | under |
| Thame United vs Eastbourne Borough 03 oct | 3 | 1.86 / 1.45 | – | – |
| Ebbsfleet United vs Sholing 03 oct | 3 | 1.76 / 1.81 | 3 | push |
| Cray Wanderers vs Chippenham Town 03 oct | 3 | 1.81 / 1.71 | – | – |
| Harborough Town vs St Albans City 03 oct | 3 | 1.78 / 1.78 | – | – |
| Rushden & Diamonds vs Braintree Town 03 oct | 3 | 1.79 / 1.91 | 3 | push |
| Crowborough vs Wimborne Rown 03 oct | 3 | 1.81 / 1.56 | – | – |
| Bury Town vs Spalding United 03 oct | 3 | 2.06 / 2.06 | – | – |
| Truro City vs Cirencester Town 03 oct | 3.5 | 2.00 / 2.00 | 4 | over |
| Mulbarton Wanderers vs Gloucester City 03 oct | 2.5 | 1.69 / 1.69 | – | – |
| Cambridge United FC vs Alvechurch 03 oct | 3 | 1.81 / 1.81 | 3 | push |
| Plymouth Parkway vs Horsham 03 oct | 3 | 2.01 / 1.97 | – | – |
| Dorking Wanderers vs Chatham Town 03 oct | 3.5 | 2.11 / 1.99 | 1 | under |
| Uxbridge vs Hungerford Town 03 oct | 2.5 | 1.86 / 1.56 | – | – |
| Tadley Calleva vs Dartford 03 oct | 3 | 1.76 / 1.63 | 3 | push |
| Weymouth vs South East Dons 03 oct | 3.5 | 1.99 / 1.99 | – | – |
| Sheppey United vs Chertsey Town 03 oct | 3 | 1.81 / 1.69 | – | – |
| Trafford vs Atherton Collieries 03 oct | 3 | 1.81 / 1.86 | 4 | over |
| Bedford Town vs Wingate y Finchley 03 oct | 3.5 | 2.09 / 2.09 | 4 | over |
| Buxton vs South Shields 03 oct | 3 | 1.78 / 1.49 | 2 | under |
| Marine vs Warrington Town 03 oct | 2.5 | 1.86 / 1.81 | 2 | under |
| Guiseley vs Bradford Park Avenue 03 oct | 3 | 1.86 / 2.36 | 1 | under |
| Leiston vs Coventry United 03 oct | 3 | 1.97 / 1.71 | 4 | over |
| Chesham United vs Real Bedford 03 oct | 3 | 1.78 / 1.40 | 7 | over |
| Hanwell Town vs Dover Athletic 03 oct | 3 | 1.81 / 1.81 | 3 | push |
| Gainsborough Trinity vs Anstey Nomads 03 oct | 3 | 1.86 / 1.97 | 5 | over |
| Barton Town vs Chorley 03 oct | 3 | 1.76 / 1.76 | 5 | over |
| Salisbury vs Dulwich Hamlet 03 oct | 3 | 1.81 / 1.89 | 5 | over |
| FC Bury vs Bromsgrove 03 oct | 3 | 1.78 / 1.74 | 3 | push |
| Kettering Town vs FC Worcester City 03 oct | 3 | 1.78 / 1.97 | 4 | over |
| Oxford City vs Enfield Town 03 oct | 3 | 1.78 / 1.60 | 7 | over |
| FC Morecambe vs Darlington 03 oct | 2.5 | 1.76 / 1.54 | 3 | over |
| Macclesfield Town vs Scarborough Athletic 03 oct | 2.5 | 1.97 / 1.69 | 4 | over |
| Redditch United vs Berkhamsted 03 oct | 2.5 | 1.74 / 1.74 | 2 | under |
| Worksop Town FC vs United of Manchester 03 oct | 3.5 | 2.06 / 1.74 | 3 | under |
| Leatherhead vs Hanworth Villa 03 oct | 3 | 1.81 / 1.81 | 1 | under |
| Spennymoor Town vs Chester 03 oct | 2.5 | 1.78 / 1.66 | 5 | over |
| Stafford Rangers vs Halesowen Town 03 oct | 2.5 | 1.61 / 2.01 | 2 | under |
| Brackley Town vs Chelmsford City 03 oct | 2.5 | 1.97 / 1.86 | 2 | under |
| Brentwood Town vs Dagenham & Redbridge 03 oct | 3 | 1.81 / 1.86 | 4 | over |
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